On Tuesday, Tomic was beaten 6-2 6-2 6-4 by Jo-Wilfried Tsonga in just 58 minutes on Court 12.
Footage of his clear lack of effort quickly spread on social media but the Australian was unapologetic about his display in his press conference.
When asked if he expected to be fined, Tomic initially gave no answer but when pressed about whether he would be bothered to lose the money, he replied: " I think I played as best as I could.
"It's just I played terrible. And he served 24 aces."
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But this morning in a statement from Wimbledon officials, tournament referee Andrew Jarrett deemed Tomic had contravened the Grand Slam Rule Book regarding first-round Performance.
The statement said: "It is the opinion of the Referee that the performance of Bernard Tomic in his first-round match against Jo-Wilfried Tsonga did not meet the required professional standards, and therefore he has been fined the maximum amount of £45,000 which will be deducted from prize money.
The rule says: "All players are expected to perform to a professional standard in every Grand Slam match.
"With respect to First Round Performance, if in the opinion of the Referee the player did not perform to the required professional standard, the Referee may determine that the player be subject to a fine of up to first-round prize money."
Speaking on the BBC, Great Britain's Fed Cup captain Keothavong didn't hide her disappointment at Tomic's display.
"This performance was an absolute disgrace," Keothavong said on the BBC.
"I just don't understand as a professional tennis player why show up and do that.
"It makes no sense whatsoever. It is just disappointing."
Tomic has the right to appeal but is highly unlikely to be successful.