The Women's Tennis Association says female players competing in singles matches will be allowed a 10-minute break between the second and third sets when temperatures go above 30.1C.
However, male players will literally have to sweat it out because the men's game does not allow for a break.
Forecasters have predicted temperatures could soar to a staggering 36C this week - with the record for the hottest day at SW19 standing at 34.6C in 1976.
Bookmakers Paddy Power are offering 2/1 that the record will be broken this year.
A spokesman for Wimbledon organisers the All-England Club said the difference in rules was due to the men's and women's games having separate governing bodies.
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I don't know why there are different rules
Top male players have previously expressed their concerns about having to continue playing during hot weather.
Last year, Britain's Andy Murray criticised the rules after 42C temperatures at the Australian Open caused a ball boy to collapse and a player to vomit.
He said: "I don't know why there are different rules.
"If there's a medical reason for it, then I'm fine with it, if there isn't, I'm not."
Former Wimbledon women's champion Maria Sharapova, who beat Australian-born British competitor Johanna Konta yesterday, admitted she did not know the rules existed but said she did not think it was unfair.
She added: "I think if it's something that they're concerned about, they can re-evaluate.
"If it does get quite hot for us, and we're able to use it, then why not?"