The Goodfellas star, who owns restaurants and a hotel in the Big Apple, met with Mayor Michael Bloomberg and other business owners in the city on Thursday (24Jun10) to promote the Signs of Support drive.
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The campaign is calling for companies in New York to publicly show their support by displaying a special sign in their doors or windows.
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The sticker reads, "We Support the 9/11 Memorial. Forever Changed. Forever Connected."
De Niro placed one of the signs in his Locanda Verde restaurant to demonstrate his support during a photocall on Thursday.
More than 2,500 of the plaques have been distributed across the city, while De Niro is also encouraging businesses to donate £67 or more to the cause.